Exp8

        RNA would most likely be found in organism with high cytoplasmic to nuclear ratio. Yeast is  a good example of this tissue.  Different kind of RNA such as mRNA, tRNA, rRNA  may require different method. From yeaast, RNA requires incubating it with alkaline solution to extract the nucleic acid and proteins that are HO soluble. This would inactivate nucleasis that would interfere with the proceeding reaction. After employing this method, RNA is obtained by acid extraction at pH 4.5 and then precipitated by the addition of acidified alcohol. Lipids may be an interfering substance and this should be removed by washings with alcohol or ether or other organic solutions.
 

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Chemistry 145.1
Human Genome Project and Bioinformatics
Experiment No. 1
Experiment No. 2
Experiment No. 3
Experiment No. 4
Experiment No. 5
Experiment No. 6
Experiment No. 7
Experiment No. 8
Experiment No. 9
Experiment No. 10
Experiment No. 11
Experiment No. 12
References